Review



freestyle 293 serum free medium  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Thermo Fisher freestyle 293 serum free medium
    Freestyle 293 Serum Free Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/Cholesterol/10__1016_slash_j__omta__2025__201654-386-6-9
    Average 97 stars, based on 1 article reviews
    freestyle 293 serum free medium - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A
    Article Snippet: .. For protein expression, HEK293F cells were cultured in 30 ml of Freestyle 293 serum-free medium (Gibco). .. For transfection of HEK293F cells, a polyethylenimine (PEI) based method (Polyscience) was used.

    Article Title: High Potency of a Bivalent Human V H Domain in SARS-CoV-2 Animal Models
    Article Snippet: .. HEK293F and expi293F were cultured in FreeStyle 293 serum free medium (ThermoFisher, Cat12338018) and Expi293 Expression Medium (ThermoFisher, Cat# A1435103), respectively. ..

    Cell Culture:

    Article Title: Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A
    Article Snippet: .. For protein expression, HEK293F cells were cultured in 30 ml of Freestyle 293 serum-free medium (Gibco). .. For transfection of HEK293F cells, a polyethylenimine (PEI) based method (Polyscience) was used.

    Article Title: High Potency of a Bivalent Human V H Domain in SARS-CoV-2 Animal Models
    Article Snippet: .. HEK293F and expi293F were cultured in FreeStyle 293 serum free medium (ThermoFisher, Cat12338018) and Expi293 Expression Medium (ThermoFisher, Cat# A1435103), respectively. ..

    Article Title: Effect of Recombinant Human Lecithin Cholesterol Acyltransferase Infusion on Lipoprotein Metabolism in Mice
    Article Snippet: The plasmid pCMV6-XL4/LCAT, encoding human LCAT cDNA, was purchased from Origene (Rockville, MD) and ligated into pcDNA3.1/Hygro (Invitrogen, Carlsbad, CA). .. Stably transfected HEK293f cells were selected with 200 μg/ml hygromycin B and grown in Freestyle 293 serum-free medium (Invitrogen) in 10-liter shake flasks for 4 days. rLCAT was isolated from conditioned cell culture medium by precipitation with zinc chloride ( Zaworski and Gill, 1988 ), followed by batch capture with phenyl-Sepharose (GE Healthcare, Little Chalfont, Buckinghamshire, UK) and batch elution, with 20 mM Tris and 0.5 M NaCl. ..

    Incubation:

    Article Title: Protein expression/secretion boost by a novel unique 21-mer cis -regulatory motif (Exin21) via mRNA stabilization
    Article Snippet: .. The culture medium was replaced with FreeStyle 293 serum free medium (Thermo Fisher Scientific, cat. no. 12-338-018) with 10 μg/mL BA and incubation was continued for 24 h at 37°C in 5% CO 2 . ..

    Article Title: Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization.
    Article Snippet: .. The culture medium was replaced with FreeStyle 293 serum free medium (Thermo Fisher Scientific, cat. no. 12-338-018) with 10 mg/mL BA and incubation was continued for 24 h at 37 C in 5% CO2. ..

    Suspension:

    Article Title: Enhanced transcriptional insulation of lentiviral vectors using “sequence-upgraded polyA long terminal repeats” or “supA-LTRs”
    Article Snippet: The safety and quality attributes of viral vector-based products are of paramount importance.. Recent successful commercialized use of lentiviral vectors (LVs) ex vivo anticipates future, higher dose applications in vivo, leading to greater integration events per patient.. Contemporary LVs employ “self-inactivating” (SIN) long terminal repeats (LTRs) that are deleted for enhancer-promoter sequences, which greatly minimizes the probability of insertional oncogenesis.

    Stable Transfection:

    Article Title: Effect of Recombinant Human Lecithin Cholesterol Acyltransferase Infusion on Lipoprotein Metabolism in Mice
    Article Snippet: The plasmid pCMV6-XL4/LCAT, encoding human LCAT cDNA, was purchased from Origene (Rockville, MD) and ligated into pcDNA3.1/Hygro (Invitrogen, Carlsbad, CA). .. Stably transfected HEK293f cells were selected with 200 μg/ml hygromycin B and grown in Freestyle 293 serum-free medium (Invitrogen) in 10-liter shake flasks for 4 days. rLCAT was isolated from conditioned cell culture medium by precipitation with zinc chloride ( Zaworski and Gill, 1988 ), followed by batch capture with phenyl-Sepharose (GE Healthcare, Little Chalfont, Buckinghamshire, UK) and batch elution, with 20 mM Tris and 0.5 M NaCl. ..

    Transfection:

    Article Title: Effect of Recombinant Human Lecithin Cholesterol Acyltransferase Infusion on Lipoprotein Metabolism in Mice
    Article Snippet: The plasmid pCMV6-XL4/LCAT, encoding human LCAT cDNA, was purchased from Origene (Rockville, MD) and ligated into pcDNA3.1/Hygro (Invitrogen, Carlsbad, CA). .. Stably transfected HEK293f cells were selected with 200 μg/ml hygromycin B and grown in Freestyle 293 serum-free medium (Invitrogen) in 10-liter shake flasks for 4 days. rLCAT was isolated from conditioned cell culture medium by precipitation with zinc chloride ( Zaworski and Gill, 1988 ), followed by batch capture with phenyl-Sepharose (GE Healthcare, Little Chalfont, Buckinghamshire, UK) and batch elution, with 20 mM Tris and 0.5 M NaCl. ..

    Isolation:

    Article Title: Effect of Recombinant Human Lecithin Cholesterol Acyltransferase Infusion on Lipoprotein Metabolism in Mice
    Article Snippet: The plasmid pCMV6-XL4/LCAT, encoding human LCAT cDNA, was purchased from Origene (Rockville, MD) and ligated into pcDNA3.1/Hygro (Invitrogen, Carlsbad, CA). .. Stably transfected HEK293f cells were selected with 200 μg/ml hygromycin B and grown in Freestyle 293 serum-free medium (Invitrogen) in 10-liter shake flasks for 4 days. rLCAT was isolated from conditioned cell culture medium by precipitation with zinc chloride ( Zaworski and Gill, 1988 ), followed by batch capture with phenyl-Sepharose (GE Healthcare, Little Chalfont, Buckinghamshire, UK) and batch elution, with 20 mM Tris and 0.5 M NaCl. ..



    Similar Products

    97
    Thermo Fisher freestyle 293 serum free medium
    Freestyle 293 Serum Free Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/Cholesterol/10__1016_slash_j__omta__2025__201654-386-6-9
    Average 97 stars, based on 1 article reviews
    freestyle 293 serum free medium - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    90
    Thermo Fisher serum-free freestyle 293 expression medium
    Serum Free Freestyle 293 Expression Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/freestyle+serum+free+medium/us12139551-530-5-10
    Average 90 stars, based on 1 article reviews
    serum-free freestyle 293 expression medium - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher freestyle™ 293 expression medium (serum free medium)
    Freestyle™ 293 Expression Medium (Serum Free Medium), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/bio_rxiv__2024__10__21__619348-225-7-1
    Average 90 stars, based on 1 article reviews
    freestyle™ 293 expression medium (serum free medium) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher 293 freestyle serum-free medium
    293 Freestyle Serum Free Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/pmc11321157-45-12-15
    Average 90 stars, based on 1 article reviews
    293 freestyle serum-free medium - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher serum-free medium freestyle 293
    Serum Free Medium Freestyle 293, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/freestyle+serum+free+medium/pm37859508-140-25-29
    Average 90 stars, based on 1 article reviews
    serum-free medium freestyle 293 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher serum free freestyle 293 expression medium
    Serum Free Freestyle 293 Expression Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/us11780919-1169-43-49
    Average 90 stars, based on 1 article reviews
    serum free freestyle 293 expression medium - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher serum-free freestyle 293 medium
    Serum Free Freestyle 293 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freestyle+293+serum+free+medium/freestyle+serum+free+medium/pmc10612092-106-13-17
    Average 90 stars, based on 1 article reviews
    serum-free freestyle 293 medium - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results